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Alcohol induced NLRP3 inflammasome activation in the brain of rats is attenuated by ATRA supplementation

Brain, Behavior, & Immunity - Health. 2020; 
S.H.PriyankaA.J.ThusharaArun A.RaufM.Indira
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Recombinant Proteins The sac was everted so that the serosal layer was inside, and the mucosal layer was outside. The sac was filled with 10??ml of Krebs’s buffer and incubated in Krebs’s buffer with endotoxin standard solution at 37??°C under O2?supply in a sterilized conical flask. The samples were injected out from the sac and endotoxin concentrations in sample were detected by means of ToxinSensor Chromogenic LAL endotoxin assay kit (Cat. No. L00350, GenScript, USA), according to manufacturer’s instructions. Get A Quote

摘要

Alcohol abuse affects several neurological pathways and causes significant alterations in the brain. Abstention from alcohol is an effective intervention against alcohol related diseases. But the recovery of the damaged cells to normal presents a major problem in those who have stopped alcohol consumption. Hence therapeutic interventions are needed. Our previous studies have shown that all trans retinoic acid (ATRA) is effective in reducing alcohol induced neuro toxicity. Chronic alcohol administration up-regulates and activates the NLRP3 inflammasome leading to caspase-1 activation and IL-1β production causing neuroinflammation. Hence, we investigated whether ATRA has any impact on NLRP3 inflammasomes activat... More

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