| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | The column was centrifuged at 8,000 RMP for 1 minute and the flow through was discarded. To the column, 600 μL of Omega DNA Wash Buffer was added and centrifuged at 8,000 RPM for a minute. This was repeated twice. The column was centrifuged at 14,000 RPM for 5 min to dry the column. The collection tube was replaced and 50 μL elution buffer was added. The column was incubated at 37°C for 15 min and centrifuged at 8,000 RPM for a minute. The DNA extraction product was collected and stored at -20°C. To a PCR tube, 46 μL of a master mix [1392 μL Sterile deionized water, 200 μL GenScript 10x PCR Buffer, 40 μL 10 μM GenScript dNTPs, 100 μL 10 μM Forward Primer Integrated DNA Technologies (IDT) SSU-bact-27f (5'-AGA GTT TGA TCM TGG CTC AG-3'), 100 μL 10 μM Reverse Primer Integrated DNA Technologies (IDT) SSUbact-519r (5′-GWA TTA CCG CGG CKG CTG-3′), and 8 μL 5 U/μL GenScript Taq DNA Polymerase] and 4 μL of DNA extraction product were added and a routine thermocycle reaction was performed. | Get A Quote |
The antimicrobial effects of silver ions and silver chloride nanoparticles have been well established while the efficacy of microcrystalline silver chloride has been less studied. Certex-AM, a microcrystalline silver chloride product produced by Cerion, Rochester, NY, was tested for its antimicrobial properties as a possible replacement for traditional biocidal techniques used in water cooling towers. The minimum inhibitory concentration (MIC) of the compound was determined using a microtiter broth assay. The compound was found to have inhibitory effects on bacterial growth for all tested organisms at concentrations greater than 9 ppm. Additional testing simulating a water cooling system showed the effectivenes... More
