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Quantitative PCR Assays Developed for Diaporthe helianthi and Diaporthe gulyae for Phomopsis Stem Canker Diagnosis and Germplasm Screening in Sunflower (Helianthus annuus)

Plant Dis. 2020; 
Elverson TR, Kontz BJ, Markell SG, Harveson RM, Mathew FM.
Products/Services Used Details Operation
Recombinant Proteins … 124 combinations were designed for D. helianthi and D. gulyae, respectively using the Real Time 125 PCR (TaqMan?) Primer Design tool (GenScript, Piscataway, NJ) and the PrimerQuest tool 126 (Integrated DNA Technologies, Coralville, IA) (data not shown) … Get A Quote

摘要

Phomopsis stem canker of sunflower is caused by two fungal pathogens, Diaporthe helianthi and Diaporthe gulyae, in the United States. In this study, two quantitative PCR (qPCR) assays were developed to detect and quantify D. helianthi and D. gulyae in sunflower. The two assays differentiated the two fungi from each other, other species of the genus Diaporthe, and pathogens, and they have high efficiency (>90%). The qPCR assays detected the two pathogens on plant samples exhibiting Phomopsis stem canker symptoms sampled from commercial sunflower fields in Minnesota, Nebraska, North Dakota, and South Dakota. Furthermore, the assays were used to screen cultivated sunflower accessions for resistance to D. helianthi... More

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