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Nanostructured recombinant protein particles raise specific antibodies against the nodavirus NNV coat protein in sole

Fish Shellfish Immunol. 2020; 
Thwaite R, Berbel C, Aparicio M, Torrealba D, Pesarrodona M, Villaverde A, Borrego JJ, Manchado M, Roher N.
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Catalog Antibody … to collect the soluble protein fraction (supernatant) Aliquots were run on a Western blot and the protein was detected using an anti-His-tag antibody (GenScript A00186-100) (Supplementary Fig S1 A) For use in ELISAs, soluble … Get A Quote

摘要

Nervous necrosis virus (NNV) reassortant strains RGNNV/SJNNV have emerged as a potent threat to the Mediterranean marine aquaculture industry, causing viral encephalopathy and retinopathy (VER) in Senegalese sole (Solea senegalensis). In this study, a cheap and practical vaccine strategy using bacterial inclusion bodies made of the coat protein of a virulent reassortant strain of this betanodavirus was devised. The nanostructured recombinant protein nanoparticles, VNNV-CNP, were administered without adjuvant to two groups of juvenile sole, one by intraperitoneal injection and the other by oral intubation. Specific antibodies were raised in vivo against the NNV coat protein via both routes, with a substantial sp... More

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