Products/Services Used | Details | Operation |
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Gene Synthesis> | The samples were separated on 4-10% SDS-PAGE gel, transferred, probed with GFP (sc-9996), CCT8 (sc-13891), CDK5 (sc-173), p35 (sc-820), ac-tubulin (6-11B-1) (Santa Cruz Biotechnology), AT8 (MN1020), ?-tubulin (DM1A) (62204) (Invitrogen), pT231 (55313), pT181 (54960) (ANASPEC), pS199 (A00894), tau (A01387), Avi (A00674), His (A00174) (GenScript), mCherry (GTX59788) (GeneTex), CCT2 (WH0010576M1) (Sigma), actin (60008-1-Ig) (Proteintech), CCT4 (EPR8495), CCT5 (EPR7562) (Epitomics), GSK3? (12456), pGSK3?(Ser9) (9323) (Cell Signaling), pGSK3?(Tyr216) (13A) (BD), HA. | Get A Quote |
The cytosolic chaperonin T-complex protein (TCP) 1-ring complex (TRiC) has been shown to exert neuroprotective effects on axonal transport through clearance of mutant Huntingtin (mHTT) in Huntington's disease. However, it is presently unknown if TRiC also has any effect on axonal transport in wild-type neurons. Here, we examined how TRiC impacted the retrograde axonal transport of brain-derived neurotrophic factor (BDNF). We found that expression of a single TRiC subunit significantly enhanced axonal transport of BDNF, leading to an increase in instantaneous velocity with a concomitant decrease in pauses for retrograde BDNF transport. The transport enhancing effect by TRiC was dependent on endogenous tau expres... More