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Intranasal vaccination with M2e5x virus-like particles induces humoral and cellular immune responses conferring cross-protection against heterosubtypic influenza viruses.

PLoS ONE. 2018; 
Lee YT, Ko EJ, Lee Y, Kim KH, Kim MC,, Lee YN,, Kang SM.
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Cellular Analysis The endotoxin levels of prepared 5xM2e VLP vaccine were determined by Chromogenic LAL endotoxin assay kit (Cat# L00350, GenScript) and found to be less than 1. Get A Quote

摘要

Current influenza vaccines do not provide broad cross-protection. Here, we report that intranasal vaccination with virus-like particles containing the highly conserved multiple ectodomains of matrix protein 2 (M2e5x VLP) of influenza virus induces broad cross-protection by M2-specific humoral and cellular immune responses. M2e5x VLP intranasal vaccination prevented severe weight loss, attenuated inflammatory cytokines and cellular infiltrates, and lowered viral loads, and induced germinal center phenotypic B and plasma cells. In addition, depletion studies demonstrate the protective roles of CD4 and CD8 T cells induced by M2e5x VLP intranasal vaccination. Thus, this study provides evidence that mucosal delivery... More

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