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Identification of microRNA?363?3p as an essential regulator of chondrocyte apoptosis in osteoarthritis by targeting NRF1 through the p53?signaling pathway

Mol Med Rep. 2020; 
Zhang M, Wang Z, Li B, Sun F, Chen A, Gong M.
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Proteins, Expression, Isolation and Analysis … No MB01015; GenScript) and incubated for 5 min A total of 40 μg of proteins was separated by 10% SDS?PAGE gel The PVDF membrane was blocked with TBS containing 5% non?fat milk and 01% Tween for 1 h at room temperature … Get A Quote

摘要

Osteoarthritis (OA) is a degenerative joint disease that affects the physical, and mental health of middle?aged and elderly people. The aims of the present study were to determine the biological function and molecular mechanisms of miR?363?3p in chondrocyte apoptosis. Exploration of the molecular mechanisms of OA may be helpful in the understand of the causes, and facilitating the prevention and treatment of OA. In the present study, the expression of nuclear respiratory factor1 (NRF1) was downregulated in the articular cartilage of OA rats in?vivo and lipopolysaccharide (LPS)?treated chondrocytes in?vitro. MicroRNAs (miRNA) are regulators of gene expression in the progression of OA. TargetScan softwa... More

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