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Mass Production of Highly Active NK Cells for Cancer Immunotherapy in a GMP Conform Perfusion Bioreactor.

Front Bioeng Biotechnol. 2019; 
Br?kerKatharina,SinelnikovEvgeny,GustavusDirk,SchumacherUdo,P?rtnerRalf,HoffmeisterHans,LüthStefan,DammermannWe
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Recombinant Proteins human IL-2 (GenScript), Get A Quote

摘要

NK cells have emerged as promising candidates for cancer immunotherapy, especially due to their ability to fight circulating tumor cells thereby preventing metastases formation. Hence several studies have been performed to generate and expand highly cytotoxic NK cells , e.g., by using specific cytokines to upregulate both their proliferation and surface expression of distinct activating receptors. Apart from an enhanced activity, application of NK cells as immunotherapeutic agent further requires sufficient cell numbers and a high purity. All these parameters depend on a variety of different factors including the starting material, additives like cytokines as well as the culture system. Here we analyz... More

關鍵詞

GMP,cytotoxicity,immunotherapy,mass production process,natural killer cells (NK cells),perfusion bioreactor,tumor immu
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