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The Drosophila Pioneer Factor Zelda Modulates the Nuclear Microenvironment of a Dorsal Target Enhancer to Potentiate Transcriptional Output.

Curr Biol. 2019-04; 
YamadaShigehiro,WhitneyPeter H,HuangShao-Kuei,EckElizabeth C,GarciaHernan G,RushlowChristi
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Recombinant Proteins . The entire MS2v5(-TAG) sequence was constructed as a G-block by GenScript, confirmed by sequencing, and incorporated into our reporter construct by Gibson Assembly (New England Biolabs, Inc.). Get A Quote

摘要

Connecting the developmental patterning of tissues to the mechanistic control of RNA polymerase II remains a long-term goal of developmental biology. Many key elements have been identified in the establishment of spatial-temporal control of transcription in the early Drosophila embryo, a model system for transcriptional regulation. The dorsal-ventral axis of the Drosophila embryo is determined by the graded distribution of Dorsal (Dl), a homolog of the nuclear factor κB (NF-κB) family of transcriptional activators found in humans [1, 2]. A second maternally deposited factor, Zelda (Zld), is uniformly distributed in the embryo and is thought to act as a pioneer factor, increasing enhancer accessibi... More

關鍵詞

Zelda,enhancer mode,microenvironment,morphogen gradient,pioneer,transcrip
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