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The MAPK pathway is involved in the regulation of rapid pacing-induced ionic channel remodeling in rat atrial myocytes.

Mol Med Rep. 2016; 
ChengWei,ZhuYun,WangHai
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Gene Synthesis PCR was run on the Veriti Thermal Cycler (Thermo Fisher Scientific, Inc.) with each sample at 20 μl, including 1 μl primer, 10 μl Taq Master mix (2X, E00019, Genscript Biotech Co., Nanjing, China) and 9 μl deionized/distilled water. Get A Quote

摘要

Alterations to the expression L?type calcium channels (LTCCs) and Kv4.3 potassium channels form the possible basis of atrial electrical remodeling during rapid pacing. The mitogen?activated protein kinase (MAPK) pathway is affected by increases in cytoplasmic Ca2+, and therefore represents an attractive candidate for the regulation and mediation of Ca2+?induced ion channel remodeling. The present study aimed to investigate alterations to the ion channel?MAPK axis, and to determine its influence on ion channel remodeling during atrial fibrillation. Rat atrial myocytes were isolated, cultured, and in vitro rapid pacing was established. Intracellular Ca2+ signals were monitored using the Fluo?3/A... More

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