| Products/Services Used | Details | Operation |
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| Recombinant Proteins | After being dialyzed against phosphate-buffered saline (PBS, containing 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4, pH 7.4), the purified protein was passed through a Detoxi-Gel? Endotoxin Removing Gel (Thermo Scientific, Waltham, MA, USA) and quantified by a ToxinSensor Chromogenic LAL Endotoxin Assay Kit (GenScript, Nanjing, China) to confirm a low level of endotoxin (<0.2 EU/mL). | Get A Quote |
Dental caries is a common disease caused by oral bacteria. and are the primary cariogenic microbes that often survive as biofilms on teeth. In this study， we evaluated the activity of ClyR， a well-known chimeric lysin with extended streptococcal host range， against common Gram-positive oral microbes and its anticaries efficacy in rat models. ClyR demonstrated high lytic activity against MT8148 and ATCC6715， with minor activity against ， ， and ， which are considered as harmless commensal oral bacteria. Confocal laser scanning microscopy showed that the number of viable cells in 72-h aged and biofilms are significantly ( < 0.05) decreased after treatment with 50 μg/mL ClyR for 5 min. Furtherm... More
