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Proteins, Expression, Isolation and Analysis> | … 2.3. Endotoxin levels of PM 2.5. Levels of endotoxin in PM 2.5 were measured using the ToxinSensor Chromogenic LAL Endotoxin Assay Kit (Cat # L00350C, GenScript, Piscataway, NJ), per manufacturer's protocol. 2.4. Treatment of cells with PM 2.5 … | Get A Quote |
Exposure to particulate matter?2.5?μm (PM) is associated with a variety of airway diseases. Although studies have demonstrated that high doses of PM cause cytotoxicity and changes to gene expression in bronchial epithelial cells, the effect of lower doses and repeated exposure to PM are less well studied. Here, we treated BEAS-2B cells with varying doses of PM for 1-7?days and examined the expression of a variety of genes implicated in airway disorders. At high doses, PM increased the expression of IL6, TNF, TSLP, CSF2, PTGS2, IL4R, and SPINK5. Other genes such as ADAM33, ORMDL3, DPP10 and CYP1A1, however, were increased by PM at much lower doses (≤1?μg/cm). Repeated ex... More
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