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A bioorthogonal probiotic platform spatiotemporally releases nanobodies in deep tumor for cancer chemoimmunotherapy

Materials Today . 2024-09; 
Ruiyue Chen , Qianglan Lu , Fei Zeng , Shurong Qin , Yongchun Pan , Zhiyong Liu , Yuzhen Wang , Bangshun He , Wenjin Qi , Yujun Song
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Recombinant Proteins nterleukin-4 (IL-4) and Granulocyte Macro-phage Colony Stimulating Factor (GM-CSF) were obtained from GenScript Biotech Corporation. All antibodies for flow cytometry analysis were used from BioLegend (USA). Get A Quote

摘要

The effectiveness of immune checkpoint blockade therapies in treating solid tumors is hindered by the limited activation of the tumor immune microenvironment within deep tumors. Immunogenic cell death offers a promising method to enhance anti-tumor immune responses. Integrating these two approaches in cancer chemoimmunotherapy presents a novel perspective. Here, a self-mineralized bioorthogonal probiotic platform with the expression of anti-PD-L1 nanobodies was developed for cancer chemoimmunotherapy. This platform selectively accumulated and deeply penetrated into tumor tissues, where the palladium-mineralized probiotics catalyzed bioorthogonal bond-cleavage to generate chemotherapeutic drugs and induced immun... More

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