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Activation of ATF3 via the integrated stress response pathway regulates innate immune response to restrict Zika virus

Virology Journal. 2024-08; 
Pheonah Badu , Gabriele Baniulyte , Morgan A Sammons , Cara T Pager
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CRISPR RNAs/Cas9 Proteins(INACTIVE) We generated A549 ATF3 KO cells in our laboratory using the CRISPR/Cas9 system. The following gRNA sequence targeting ATF3 was cloned into pLentiCRISPRv2 plasmid: 5′-CCACCGGATGTCCTCTGCGC-3′ (Genscript, Clone ID C88007). Get A Quote

摘要

Zika virus (ZIKV) is a re-emerging mosquito-borne flavivirus that can have devastating health consequences. The developmental and neurological effects of a ZIKV infection arise in part from the virus triggering cellular stress pathways and perturbing transcriptional programs. To date, the underlying mechanisms of transcriptional control directing viral restriction and virus-host interaction are understudied. Activating Transcription Factor 3 (ATF3) is a stress-induced transcriptional effector that modulates the expression of genes involved in a myriad of cellular processes, including inflammation and antiviral responses, to restore cellular homeostasis. While ATF3 is known to be upregulated during ZIKV infectio... More

關(guān)鍵詞

Zika virus; flavivirus; innate immune response; integrated stress response; transcription factor.
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