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Combined biolistic and cell penetrating peptide delivery for the development of scalable intradermal DNA vaccines

J Control Release. 2024-01; 
Roizza Beth So, Gang Li, Victoria Brentville, Janet M Daly, James E Dixon
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Proteins, Expression, Isolation and Analysis … All samples were added in triplicates into high protein-binding ELISA plates (Nunc Immuno F96 MaxiSorp, Thermo Fisher) that were pre-coated with S1 protein (GenScript, UK) at 200 ng… Get A Quote

摘要

Physical-based gene delivery via biolistic methods (such as the Helios gene gun) involve precipitation of nucleic acids onto microparticles and direct transfection through cell membranes of exposed tissue (e.g. skin) by high velocity acceleration. The glycosaminoglycan (GAG)-binding enhanced transduction (GET) system exploits novel fusion peptides consisting of cell-binding, nucleic acid condensing, and cell-penetrating domains, which enable enhanced transfection across multiple cell types. In this study, we combined chemical (GET) and physical (gene gun) DNA delivery systems, and hypothesized the combination would generate enhanced distribution and effective uptake in cells not initially transfected by biolist... More

關(guān)鍵詞

Cell penetrating peptide (CPP), DNA vaccine, GAG-binding enhanced transduction (GET), Gene delivery, Gene gun, SARS-CoV-2
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