| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … An amount of 15 μL of the supernatant was loaded onto a gradient SDS-polyacrylamide gel (GenScript, Nanjing, China. Cat. M00654) and electrophoresed at 120 V in regular Tris-… | Get A Quote |
Cytidine triphosphate synthase (CTPS) forms cytoophidia in all three domains of life. Here we focus on the function of cytoophidia in cell proliferation using as a model system. We find that converting His of CTPS into Ala leads to cytoophidium disassembly. By reducing the level of CTPS protein or specific mutation, the loss of cytoophidia prolongs the G2 phase and expands cell size. In addition, the loss-filament mutant of CTPS leads to a decrease in the expression of genes related to G2/M transition and cell growth, including histone chaperone . The overexpression of alleviates the G2 phase elongation and cell size enlargement induced by CTPS loss-filament mutants. Overall, our results connect cytoophidia w... More
