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Development of nanobodies specific to clumping factors A of Staphylococcus aureus by yeast surface display

Int J Biol Macromol. 2024-01; 
Meng Mei, Mengqing Lu, Shiqi Li, Xinyi Ren, Banbin Xing, Yang Hu, Yuqi Wu, Huan Chen, Longhao Wang, Li Yi, Ke Ming, Zigong Wei
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Catalog Antibody … (GenScript, Nanjing, China) for 15 min in dark. The antibody-labeled cells were resuspended in 1× PBS buffer and sorted using Beckman Coulter MoFlo XDP flow cytometer (Brea, CA, … Get A Quote

摘要

The Staphylococcus aureus clumping factor A (ClfA) is a fibrinogen (Fg) binding protein that plays an important role in the clumping of S. aureus in blood plasma. The current anti-infective approaches targeting ClfA are mainly based on monoclonal antibodies but showed less impressive efficacy for clinical applications. Nanobodies offer advantages in enhanced tissue penetration and a propensity to bind small epitopes. However, there is no report on generating specific nanobodies for ClfA. Here, we constructed a synthetic nanobody library based on yeast surface display to isolate nanobodies against the Fg binding domain ClfA. We firstly obtained a primary nanobody directed to ClfA, and then employed error-prone m... More

關鍵詞

ClfA, Fibrinogen, Nanobody
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