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Enhancing CAR Macrophage Efferocytosis Via Surface Engineered Lipid Nanoparticles Targeting LXR Signaling

Adv Mater. 2024-02; 
Skylar T Chuang, Joshua B Stein, Sarah Nevins, Cemile Kilic Bektas, Hye Kyu Choi, Wan-Kyu Ko, Hyunjun Jang, Jihun Ha, Ki-Bum Lee
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Proteins, Expression, Isolation and Analysis … Protein L, which recognizes immunoglobulin light chains and scFvs.[34] We chose Protein L … were stained with 1 μg of biotinylated protein L (GenScript, Piscataway, NJ) for 45 min at … Get A Quote

摘要

The removal of dying cells, or efferocytosis, is an indispensable part of resolving inflammation. However, the inflammatory microenvironment of the atherosclerotic plaque frequently affects the biology of both apoptotic cells and resident phagocytes, rendering efferocytosis dysfunctional. To overcome this problem, a chimeric antigen receptor (CAR) macrophage that can target and engulf phagocytosis-resistant apoptotic cells expressing CD47 is developed. In both normal and inflammatory circumstances, CAR macrophages exhibit activity equivalent to antibody blockage. The surface of CAR macrophages is modified with reactive oxygen species (ROS)-responsive therapeutic nanoparticles targeting the liver X receptor path... More

關鍵詞

CAR macrophage, atherosclerosis, efferocytosis, lipid nanoparticle, β‐cyclodextrin
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