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Context dependent activity of p63-bound gene regulatory elements

biorxiv. 2024-05; 
Abby A McCann, Gabriele Baniulyte, Dana L Woodstock, Morgan A Sammons
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Gene Synthesis … a range of cellular and genetic contexts to investigate the influence … Our analysis of p63-bound gene regulatory elements provides … was synthesized by GenScript (Piscataway, NJ, USA). … Get A Quote

摘要

The p53 family of transcription factors regulate numerous organismal processes including the development of skin and limbs, ciliogenesis, and preservation of genetic integrity and tumor suppression. p53 family members control these processes and gene expression networks through engagement with DNA sequences within gene regulatory elements. Whereas p53 binding to its cognate recognition sequence is strongly associated with transcriptional activation, p63 can mediate both activation and repression. How the DNA sequence of p63-bound gene regulatory elements is linked to these varied activities is not yet understood. Here, we use massively parallel reporter assays (MPRA) in a range of cellular and genetic contexts ... More

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