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A pattern recognition receptor interleukin-1 receptor is involved in reproductive immunity in Macrobrachium nipponense ovary

Fish Shellfish Immunol. 2024-03; 
Haifu Wan, Lei Yu, Xiaodong Cui, Shuai Guo, Shumei Mu, Xianjiang Kang
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Recombinant Proteins … the recombinant protein (rIG). The rIG was purified using Ni-NTA Agarose (GenScript, China) … Then, the proteins were refolded in gradient urea-TSB glycerol buffer (50 mmol L ?1 Tris-… Get A Quote

摘要

The family of TIR domain-containing receptors includes numerous proteins involved in innate immunity. In this study, a member of this family was characterized from the ovary of the oriental river prawn Macrobrachium nipponense and identified as interleukin-1 receptor (MnIL-1R). Meanwhile, to elucidate the conservation of IL-1R, its orthologous were identified in several crustacean species as well. In addition, the expression pattern of MnIL-1R in various adult tissues and post different pathogen-associated molecular patterns (PAMPs) challenge in ovary was analyzed with qRT-PCR technology. Finally, the roles of MnIL-1R in the ovary were analyzed by RNAi technology. The main results are as follows: (1) MnIL-1R co... More

關鍵詞

Macrobrachium nipponense, MnIL-1R, Ovary, Pattern recognition receptor
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