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Nintedanib downregulates the profibrotic M2 phenotype in cultured monocyte-derived macrophages obtained from systemic sclerosis patients affected by interstitial lung disease

Arthritis Res Ther. 2024-03; 
Stefano Soldano, Vanessa Smith, Paola Montagna, Emanuele Gotelli, Rosanna Campitiello, Carmen Pizzorni, Sabrina Paolino, Alberto Sulli, Andrea Cere, Maurizio Cutolo
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Proteins, Expression, Isolation and Analysis … Subsequently, 20 μg of protein was separated via electrophoresis on pre-cast 4–20% gradient tris-glycine gels (GenScript, Piscataway, USA) and then transferred onto a nitrocellulose … Get A Quote

摘要

background: Systemic sclerosis (SSc) is an autoimmune connective tissue disease characterized by vasculopathy and progressive fibrosis of skin and several internal organs, including lungs. Macrophages are the main cells involved in the immune-inflammatory damage of skin and lungs, and alternatively activated (M2) macrophages seem to have a profibrotic role through the release of profibrotic cytokines (IL10) and growth factors (TGFβ1). Nintedanib is a tyrosine kinase inhibitor targeting several fibrotic mediators and it is approved for the treatment of SSc-related interstitial lung disease (ILD). The study aimed to evaluate the effect of nintedanib in downregulating the profibrotic M2 phenotype in cultured mono... More

關(guān)鍵詞

Alternatively activated macrophages, Fibrosis, Interstitial lung disease, Systemic sclerosis, Tyrosine kinases inhibitor
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