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A novel biomarker of MMP-cleaved cartilage intermediate layer protein-1 is elevated in patients with rheumatoid arthritis, ankylosing spondylitis and osteoarthritis

Sci Rep. 2023-12; 
Helena Port, Cecilie M?ller Hausgaard, Yi He, Walter P Maksymowych, Stephanie Wichuk, Dovile Sinkeviciute, Anne-Christine Bay-Jensen, Signe Holm Nielsen
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Proteins, Expression, Isolation and Analysis … proteins, such as the cartilage intermediate layer protein (CILP… by low-turnover remodelling of matrix proteins 3 . A hallmark of … purchased from Genscript (Piscataway, NJ, US) (Table 4). … Get A Quote

摘要

Rheumatic joints have an altered cartilage turnover. Cartilage intermediate layer protein 1 (CILP-1) is secreted from articular chondrocytes and deposited into the cartilage extracellular matrix. We developed an immunoassay targeting a Matrix Metalloproteinase (MMP)-generated neo-epitope of CILP-1, named CILP-M. Human articular cartilage was cleaved with proteolytic enzymes and CILP-M levels were measured. We also quantified CILP-M in two studies from patients with rheumatoid arthritis (RA), ankylosing spondylitis (AS) and osteoarthritis (OA) and explored the monitoring and prognostic potential of CILP-M in TNF-α inhibitory treatment and modified Stoke AS Spine Score (mSASSS) progression. CILP-M was generated ... More

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