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Identification of sucrose synthase from to favor biocatalytic glycosylation

Front Microbiol. 2023-08; 
Kai Chen, Lei Lin, Ruiqi Ma, Jiajie Ding, Huayi Pan, Yehui Tao, Yan Li, Honghua Jia
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Proteins, Expression, Isolation and Analysis … the restriction endonuclease sites NcoI and EcoRI by GenScript (Nanjing, China). A 6-… resin (L00683, GenScript, Nanjing, China). The recombination proteins were eluted from the … Get A Quote

摘要

Sucrose synthase (SuSy, EC 2.4.1.13) is a unique glycosyltransferase (GT) for developing cost-effective glycosylation processes. Up to now, some SuSys derived from plants and bacteria have been used to recycle uridine 5'-diphosphate glucose in the reactions catalyzed by Leloir GTs. In this study, after sequence mining and experimental verification, a SuSy from (SuSy), a single-cell green alga, was overexpressed in , and its enzymatic properties were characterized. In the direction of sucrose cleavage, the specific activity of the recombinant SuSy is 9.39 U/mg at 37°C and pH 7.0, and the optimum temperature and pH were 60°C and pH 7.0, respectively. Its nucleotide preference for uridine 5'-diphosphate (UDP) w... More

關鍵詞

Micractinium conductrix, biotransformation, glycosylation, glycosyltransferase, rebaudioside M, sucrose synthase
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