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Cryo-EM structure of the human Asc-1 transporter complex

Nat Commun. 2024-04; 
Yaning Li , Yingying Guo, Angelika Br?er, Lu Dai, Stefan Br?er , Renhong Yan
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Anti-DYKDDDDK G1 Affinity Resin The resulting supernatant was then applied to anti-FLAG M2 affinity resin(Genscript). Get A Quote

摘要

The Alanine-Serine-Cysteine transporter 1 (Asc-1 or SLC7A10) forms a crucial heterodimeric transporter complex with 4F2hc (SLC3A2) through a covalent disulfide bridge. This complex enables the sodium-independent transport of small neutral amino acids, including L-Alanine (L-Ala), Glycine (Gly), and D-Serine (D-Ser), within the central nervous system (CNS). D-Ser and Gly are two key endogenous glutamate co-agonists that activate N-methyl-d-aspartate (NMDA) receptors by binding to the allosteric site. Mice deficient in Asc-1 display severe symptoms such as tremors, ataxia, and seizures, leading to early postnatal death. Despite its physiological importance, the functional mechanism of the Asc-1-4F2hc complex has ... More

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