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Endotoxin contamination in commercially available Cas9 proteins potentially induces T-cell mediated responses

Gene Ther. 2021-11; 
Vijaya L Simhadri, Joseph R McGill, Zuben E Sauna
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Proteins, Expression, Isolation and Analysis … licensure it is imperative the reagents are free of impurities that … The ToxinEraser TM Endotoxin Removal Resin (Genscript) … which did not meet the false discovery rate cutoff of alpha = … Get A Quote

摘要

Immune responses to Cas proteins have been demonstrated recently and these may prove to be an impediment to their clinical use in gene editing. To make meaningful assessments of Cas9 immunogenicity during drug development and licensure it is imperative the reagents are free of impurities that could affect in vitro assessments of immunogenicity. Here we address the issue of endotoxin levels in laboratory grade Cas9 proteins used to measure T-cell memory responses. Many of these reagents have not been developed for immunogenicity assays, are or microbial origin and carry varying levels of endotoxin. The use of these reagents, off the shelf, without measuring endotoxin levels is likely to introduce incorrect estim... More

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