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Synthesis and Evaluation of Clinically Translatable Targeted Microbubbles Using a Microfluidic Device for In Vivo Ultrasound Molecular Imaging

Int J Mol Sci. 2023-05; 
Rakesh Bam, Arutselvan Natarajan, Farbod Tabesh, Ramasamy Paulmurugan, Jeremy J Dahl
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Recombinant Proteins … coli (NEB) cells were used for protein purification. The bacterial colonies were outgrown in … off from the ABY protein by recombinant bovine enterokinase enzyme (GenScript, Piscataway, … Get A Quote

摘要

The main aim of this study is to synthesize contrast microbubbles (MB) functionalized with engineered protein ligands using a microfluidic device to target breast cancer specific vascular B7-H3 receptor in vivo for diagnostic ultrasound imaging. We used a high-affinity affibody (ABY) selected against human/mouse B7-H3 receptor for engineering targeted MBs (TMBs). We introduced a C-terminal cysteine residue to this ABY ligand for facilitating site-specific conjugation to DSPE-PEG-2K-maleimide (M. Wt = 2.9416 kDa) phospholipid for MB formulation. We optimized the reaction conditions of bioconjugations and applied it for microfluidic based synthesis of TMBs using DSPE-PEG-ABY and DPPC liposomes (5:95 mole %). The ... More

關(guān)鍵詞

bioconjugation, microbubbles, microfluidics, phospholipids, thiol–maleimide
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