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Acid phosphatase-like proteins, a biogenic amine and leukotriene-binding salivary protein family from the flea Xenopsylla cheopis

Commun Biol. 2023-12; 
Stephen Lu, John F Andersen, Christopher F Bosio, B Joseph Hinnebusch, José M Ribeiro
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Recombinant Proteins … (+) vector (GenScript). The recombinant acid phosphatases were produced in the HEK293 cell line and protein purification was performed using an ?KTA Pure system (GE) as follows: … Get A Quote

摘要

The salivary glands of hematophagous arthropods contain pharmacologically active molecules that interfere with host hemostasis and immune responses, favoring blood acquisition and pathogen transmission. Exploration of the salivary gland composition of the rat flea, Xenopsylla cheopis, revealed several abundant acid phosphatase-like proteins whose sequences lacked one or two of their presumed catalytic residues. In this study, we undertook a comprehensive characterization of the tree most abundant X. cheopis salivary acid phosphatase-like proteins. Our findings indicate that the three recombinant proteins lacked the anticipated catalytic activity and instead, displayed the ability to bind different biogenic amin... More

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