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An evolutionarily conserved serine protease network mediates melanization and Toll activation in

Sci Adv. 2023-12; 
Tisheng Shan, Yang Wang, Krishna Bhattarai, Haobo Jiang
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Proteins, Expression, Isolation and Analysis … Collected fractions from the Q column or DS column were subjected to 10% SDS-PAGE and immunoblotting using anti-6×His antibody (GenScript). Fractions containing the target … Get A Quote

摘要

Melanization and Toll pathway activation are essential innate immune mechanisms in insects, which result in the generation of reactive compounds and antimicrobial peptides, respectively, to kill pathogens. These two processes are mediated by phenoloxidase (PO) and Sp?tzle (Spz) through an extracellular network of serine proteases. While some proteases have been identified in in genetic studies, the exact order of proteolytic activation events remains controversial. Here, we reconstituted the serine protease framework in by biochemical methods. This system comprises 10 proteases, i.e., ModSP, cSP48, Grass, Psh, Hayan-PA, Hayan-PB, Sp7, MP1, SPE and Ser7, which form cascade pathways that recognize microbial mo... More

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