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Preclinical efficacy of a cell division protein candidate gonococcal vaccine identified by artificial intelligence

MBio. 2023-10; 
Sunita Gulati, Andreas Holm Mattsson, Sophie Schussek, Bo Zheng, Rosane B DeOliveira, Jutamas Shaughnessy, Lisa A Lewis, Peter A Rice, P?r Comstedt, Sanjay Ram
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis … antigens identified by EDEN for their efficacy against N. gonorrhoeae and have identified two novel antigens … coli strain BL21 (DE3) and purified at either GenScript or Creative Biomart. … Get A Quote

摘要

A safe and effective vaccine is urgently needed to combat the global threat of multidrug-resistant (MDR) . We screened 26 gonococcal proteins discovered by an artificial intelligence-driven platform called Efficacy Discriminative Educated Network (EDEN) trained to identify novel, protective vaccine antigens against pathogenic bacteria for efficacy in the mouse vaginal colonization model of gonorrhea. Combinations of two to three antigens adjuvanted with GLA-SE (induces T1 responses) yielded 11 groups that were used to vaccinate mice. An inverse correlation was noted between the complement-dependent bactericidal activity of antisera from each of the 11 groups and the burden of gonococcal colonization. The combin... More

關鍵詞

Neisseria gonorrhoeae, artificial intelligence, complement, vaccine
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