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Immunoaffinity monoliths for multiplexed extraction of preterm birth biomarkers from human blood serum in 3D printed microfluidic devices

Analyst. 2022-02; 
Haifa M Almughamsi, Makella K Howell, Samuel R Parry, Joule E Esene, Jacob B Nielsen, Gregory P Nordin, Adam T Woolley
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Recombinant Proteins … Corticotropin releasing factor (CRF) came from GenScript (Piscataway, NJ), and tumor necrosis factor-α receptor type 1 (TNF) was purchased from ProSpec (East Brunswick, NJ)?… Get A Quote

摘要

In an effort to develop biomarker-based diagnostics for preterm birth (PTB) risk, we created 3D printed microfluidic devices with multiplexed immunoaffinity monoliths to selectively extract multiple PTB biomarkers. The equilibrium dissociation constant for each monoclonal antibody toward its target PTB biomarker was determined. We confirmed the covalent attachment of three different individual antibodies to affinity monoliths using fluorescence imaging. Three different PTB biomarkers were successfully extracted from human blood serum using their respective single-antibody columns. Selective binding of each antibody toward its target biomarker was observed. Finally, we extracted and eluted three PTB biomarkers f... More

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