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Ratiometric Imaging of Mitochondrial Hydrogen Peroxide in Aβ-Mediated Neurotoxicity

ACS Sens. 2022-02; 
Xinyu Li, Yiyu Zhang, Hui-Wang Ai
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Mammalian Expression … well. The recipe of the brain slice culture media was identical to the reported one. (39) To treat the slices, human Aβ 42 (GenScript, Catalog no. RP10017?… Get A Quote

摘要

Oxidative stress is important for the etiology and pathogenesis of Alzheimer's disease (AD). Research tools that can conveniently evaluate oxidative stress in AD models are expected to catalyze and accelerate research on AD. This study explored the use of genetically encoded fluorescent indicators (GEFIs) to detect mitochondrial oxidative stress in organotypic brain slices and AD mouse models. To enable ratiometric normalization and avoid tissue autofluorescence, we genetically fused a green fluorescent hydrogen peroxide (HO) indicator, HyPer7, with each of two selected, bright red fluorescent proteins (RFPs), mScarlet-I and tdTomato. The resultant indicators, namely, HyPerGR and HyPerGR, were tagged with mitoc... More

關(guān)鍵詞

Alzheimer’s disease, fluorescent proteins, genetically encoded fluorescent indicators, oxidative stress, ratiometric imaging
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