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Metabolic radiolabeling and in vivo PET imaging of cytotoxic T lymphocytes to guide combination adoptive cell transfer cancer therapy

J Nanobiotechnology. 2021-06; 
Dehua Lu, Yanpu Wang, Ting Zhang, Feng Wang, Kui Li, Shixin Zhou, Hua Zhu, Zhi Yang, Zhaofei Liu
Products/Services Used Details Operation
Recombinant Proteins IL-2 (1500?IU/mL) (Genscript, Nanjing, China), Get A Quote

摘要

background: Adoptive T cell transfer-based immunotherapy yields unsatisfactory results in the treatment of solid tumors, partially owing to limited tumor infiltration and the immunosuppressive microenvironment in solid tumors. Therefore, strategies for the noninvasive tracking of adoptive T cells are critical for monitoring tumor infiltration and for guiding the development of novel combination therapies. methods: We developed a radiolabeling method for cytotoxic T lymphocytes (CTLs) that comprises metabolically labeling the cell surface glycans with azidosugars and then covalently conjugating them with Cu-1,4,7-triazacyclononanetriacetic acid-dibenzo-cyclooctyne (Cu-NOTA-DBCO) using bioorthogonal chemistry. Cu... More

關鍵詞

Adoptive cell transfer, Focal adhesion kinase inhibition, Image-guided therapy, Positron emission tomography, Radiolabeling
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