国产精品久久久久久永久牛牛,55国产福利在线视频,成人午夜精品视频在线观看

至今,GenScript的服務及產品已被Cell, Nature, Science, PNAS等1300多家生物醫藥類雜志引用近萬次,處于行業領先水平。NIH、哈佛、耶魯、斯坦福、普林斯頓、杜克大學等約400家全球著名機構使用GenScript的基因合成、多肽服務、抗體服務和蛋白服務等成功地發表科研成果,再次證明GenScript 有能力幫助業內科學家Make research easy.

CRISPR-mediated Labeling of Cells in Chick Embryos Based on Selectively Expressed Genes

Bio Protoc. 2021-08; 
Masahito Yamagata, Joshua R Sanes
Products/Services Used Details Operation
Recombinant Proteins Csa9 nuclease from GenScript Get A Quote

摘要

The abilities to mark and manipulate specific cell types are essential for an increasing number of functional, structural, molecular, and developmental analyses in model organisms. In a few species, this can be accomplished by germline transgenesis; in other species, other methods are needed to selectively label somatic cells based on the genes that they express. Here, we describe a method for CRISPR-based somatic integration of reporters or Cre recombinase into specific genes in the chick genome, followed by visualization of cells in the retina and midbrain. Loci are chosen based on an RNA-seq-based cell atlas. Reporters can be soluble to visualize the morphology of individual cells or appended to the encoded ... More

關鍵詞

CRISPR, Chick embryo, Cre, Electroporation, GFP, Homologous recombination
              主站蜘蛛池模板: 丰镇市| 临洮县| 平利县| 犍为县| 探索| 美姑县| 肇庆市| 本溪| 景洪市| 浑源县| 贵港市| 乌恰县| 内黄县| 定日县| 济源市| 林周县| 乌兰浩特市| 商丘市| 庆阳市| 长治县| 分宜县| 大方县| 溆浦县| 鄂尔多斯市| 信宜市| 涿鹿县| 原阳县| 芦溪县| 东辽县| 开阳县| 吉林市| 繁峙县| 康定县| 北川| 额济纳旗| 英山县| 白朗县| 盐山县| 石狮市| 宜君县| 循化|