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Epitope Profiling Reveals the Critical Antigenic Determinants in SARS-CoV-2 RBD-Based Antigen

Front Immunol. 2021-09; 
Min Jiang, Gaiping Zhang, Hongliang Liu, Peiyang Ding, Yunchao Liu, Yuanyuan Tian, Yanwei Wang, Aiping Wang
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Recombinant Proteins The neutralization capacity of these mAbs was assessed with a commercial SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) kit (GenScript, Nanjing, China) Get A Quote

摘要

The ongoing COVID-19 pandemic caused by SARS-CoV-2 is a huge public health crisis for the globe. The receptor-binding domain (RBD) of SARS-CoV-2 spike (S) protein plays a vital role in viral infection and serves as a major target for developing neutralizing antibodies. In this study, the antibody response to the RBD of SARS-CoV-2 S protein was analyzed by a panel of sera from animals immunized with RBD-based antigens and four linear B-cell epitope peptides (R345, R405, R450 and R465) were revealed. The immunogenicity of three immunodominant peptides (R345, R405, R465) was further accessed by peptide immunization in mice, and all of them could induced potent antibody response to SARS-CoV-2 S protein, indicating ... More

關鍵詞

RBD, SARS-CoV-2, epitope, monoclonal antibody, spike protein
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