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The Development of Dual Vaccines against Lumpy Skin Disease (LSD) and Bovine Ephemeral Fever (BEF)

Vaccines (Basel). 2021-10; 
Nicola Douglass, Ruzaiq Omar, Henry Munyanduki, Akiko Suzuki, Warren de Moor, Paidamwoyo Mutowembwa, Alri Pretorius, Tshifhiwa Nefefe, Antoinette van Schalkwyk, Pravesh Kara, Livio Heath, Anna-Lise Williamson
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摘要

Dual vaccines ( = 6) against both lumpy skin disease (LSD) and bovine ephemeral fever (BEF) were constructed, based on the BEFV glycoprotein (G) gene, with or without the BEFV matrix (M) protein gene, inserted into one of two different LSDV backbones, nLSDV?SOD-UCT or nLSDVSODis-UCT. The inserted gene cassettes were confirmed by PCR; and BEFV protein was shown to be expressed by immunofluorescence. The candidate dual vaccines were initially tested in a rabbit model; neutralization assays using the South African BEFV vaccine (B-Phemeral) strain showed an African consensus G protein gene (Gb) to give superior neutralization compared to the Australian (Ga) gene. The two LSDV backbones expressing both Gb and M BE... More

關鍵詞

LSDV challenge, bovine ephemeral fever virus, dual vaccine, lumpy skin disease virus, neutralization
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