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Preclinical immunogenicity and efficacy of a candidate COVID-19 vaccine based on a vesicular stomatitis virus-SARS-CoV-2 chimera

EBioMedicine. 2022-07; 
Amy S Espeseth, Maoli Yuan, Michael Citron, Lucia Reiserova, Gavin Morrow, Aaron Wilson, Melanie Horton, Mark Rukhman, Keith Kinek, Fuxiang Hou, Shui L Li, Fengsheng Li, Yesle Choi, Gwen Heidecker, Bin Luo, Guoxin Wu, Lan Zhang, Erica Strable, Joanne DeStefano, Susan Secore, Tarit K Mukhopadhyay, Douglas D Richardson, Eddy Sayeed, Lisa S Welch, Andrew J Bett, Mark B Feinberg, Swati B Gupta, Christopher L Cooper, Christopher L Parks
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Proteins, Expression, Isolation and Analysis … synthesised by GenScript, Inc. … Purified virus particles for Western blot analysis were prepared by collecting serum-free medium supernatant from infected Vero cell cultures and purifying … Get A Quote

摘要

background: To investigate a vaccine technology with potential to protect against coronavirus disease 2019 (COVID-19) and reduce transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with a single vaccine dose, we developed a SARS-CoV-2 candidate vaccine using the live vesicular stomatitis virus (VSV) chimeric virus approach previously used to develop a licensed Ebola virus vaccine. methods: We generated a replication-competent chimeric VSV-SARS-CoV-2 vaccine candidate by replacing the VSV glycoprotein (G) gene with coding sequence for the SARS-CoV-2 Spike glycoprotein (S). Immunogenicity of the lead vaccine candidate (VSV?G-SARS-CoV-2) was evaluated in cotton rats and golden Syrian ham... More

關鍵詞

Cotton rat, Mucosal vaccination, SARS-CoV-2 vaccine, Syrian hamster, VSV, Vesicular stomatitis virus
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