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Development of rSs-NIE-1 and rSs-IR Recombinant Antigen-Based Immunoblot for Detection of Antibody to Strongyloides stercoralis

Am J Trop Med Hyg. 2021-04; 
Joelma Nascimento De Souza, I'Isha Langford, Yong Wang, Neci Matos Soares, Sukwan Handali
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Monoclonal Antibody Services … The primary antibody for Western blot was mouse‐anti‐GST monoclonal antibody (GenScript, catalog no. A00186, GenScript, Piscataway, NJ). The concentration was determined by … Get A Quote

摘要

Strongyloides stercoralis is a soil-transmitted nematode that can cause life-threatening conditions in immunocompromised persons. In the United States, strongyloidiasis should be considered mainly in immigrants, refugees, or travelers. The confirmatory laboratory diagnosis is usually performed by detecting larvae from the stool, duodenal material, and sputum. In persons who are immunocompromised with severe strongyloidiasis, adult worms and eggs can be detected from duodenal material. For serological diagnosis, most assays use crude antigens to detect anti-S. stercoralis IgG. Recently, recombinant proteins such as rSs-NIE-1 and rSs-IR have been used to detect IgG antibodies. We used rSs-NIE-1 and rSs-IR recombi... More

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