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TNF-α stimulation enhances the neuroprotective effects of gingival MSCs derived exosomes in retinal ischemia-reperfusion injury via the MEG3/miR-21a-5p axis

Biomaterials. 2022-03; 
Ziyu Yu, Yuwen Wen, Nan Jiang, Zhidong Li, Jieying Guan, Yingying Zhang, Caibing Deng, Ling Zhao, Song Guo Zheng, Yingting Zhu, Wenru Su, Yehong Zhuo
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Proteins, Expression, Isolation and Analysis … Exosomal proteins were separated on SDS-PAGE gels (GenScript, China) and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Germany). Protein bands were … Get A Quote

摘要

Retinal ischemia-reperfusion injury (IRI) is one of the main pathogenic mechanisms of glaucoma, which are largely unknown, including neuroinflammation and neuronal death in the pathological process. In our previous studies, mesenchymal stem cells (MSCs) have been reported to play anti-inflammatory and neuroprotective roles. Additionally, conditioned culture medium (CM) of MSCs stimulated by TNF-α have achieved better antiallergic effects in an experimental allergic conjunctivitis mouse model. However, there is an urgent need for cell-free therapy approaches, like exosomes, to reduce the side effects of autoimmunity. The present study aimed to elucidate the pathways involving TNF-α-stimulated gingival MSC (GMS... More

關鍵詞

Exosomes, Gingival mesenchymal stem cells, Glaucoma, Long non-coding RNAs, Micro-RNAs, Retinal ischemia-reperfusion injury
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