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Low expression of FXYD5 reverses the cisplatin resistance of epithelial ovarian cancer cells

Histol Histopathol. 2021-02; 
Ya-Kun Liu, Ya-Jing Jia, Shi-Hao Liu, Hong-Jie Shi, Jing Ma
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Recombinant Proteins … Dallas, TX, USA) in siRNA transfection medium (Cat. #: sc-36868, Santa Cruz Biotechnology, Inc., 128 Dallas, TX, USA)?… Primers were 136 designed with Primer 5.0 software (Table 1) and synthesized by GenScript Biotech Corporation 137 (Nanjing, China)?… Get A Quote

摘要

objective: To investigate the effect of the downregulation of FXYD domain-containing ion transport regulator 5 (FXYD5) on the cisplatin resistance (CisR) of epithelial ovarian cancer (EOC) cells. methods: A2780-CisR and SKOV3-CisR cells were obtained through repeated administrations of different cisplatin concentrations, and the half-maximal inhibition concentration (IC50) was calculated by MTT assays. After transfection with FXYD5 siRNA-1 and FXYD5 siRNA-2, the IC50 values of the A2780-CisR and SKOV3-CisR cells were also detected by the MTT method. Cell proliferation, migration, invasion and apoptosis were evaluated through 5-ethynyl-2'-deoxyuridine (EdU) DNA synthesis, wound healing, Transwell invasion and An... More

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