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Berberine inhibits the proliferation, invasion and migration of endometrial stromal cells by downregulating miR?429

Mol Med Rep. 2021-04; 
Yongjuan Gu, Zhigang Zhou
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Recombinant Proteins … PCR System (Thermo Fisher Scientific, Inc.), according to the manufacturer's protocols. The amplification conditions were as follows: 95?C for 10 min, followed by 40 cycles of 95?C for 10 sec and 60?C for 60 sec. Primer sequences used in PCR were obtained from GenScript?… Get A Quote

摘要

Endometriosis (EM) is a common gynecological disease, and its pathological process is accompanied by the migration and proliferation of uterine cells. Berberine (BBR) has been shown to exhibit antitumor activity; however, the effects of BBR on EM have seldom been reported to date. The expression of microRNA (miR)?429 is upregulated in EM and miR?429 can be used as a target for drug regulation of cancer cells. Whether BBR plays a regulatory role in EM by targeting miR?429 has not been reported. Thus, the aim of the present study was to determine the effects of BBR on EM cells. The survival rate of immortalized human endometrial stromal cells (HESCs) was determined using a Cell Counting Kit?8 assay. A col... More

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