国产精品久久久久久永久牛牛,55国产福利在线视频,成人午夜精品视频在线观看

至今,GenScript的服務及產品已被Cell, Nature, Science, PNAS等1300多家生物醫藥類雜志引用近萬次,處于行業領先水平。NIH、哈佛、耶魯、斯坦福、普林斯頓、杜克大學等約400家全球著名機構使用GenScript的基因合成、多肽服務、抗體服務和蛋白服務等成功地發表科研成果,再次證明GenScript 有能力幫助業內科學家Make research easy.

A New Method for Studying RNA-binding Proteins on Specific RNAs

Bio Protoc. 2021-05; 
Weiping Sun, Ziheng Zhang, Ji-Long Liu, Min Zhuang
Products/Services Used Details Operation
Recombinant Proteins … SurePAGE (GenScript, catalog number: M00657)?… This work has been supported by Shanghai Municipal Science and Technology Commission (19JC1413700 to MZ) and National Natural Science Foundation of China (31922038 to MZ; 31771490 to JLL). Competing interests?… Get A Quote

摘要

Proximity-based protein labeling has been developed to identify protein-nucleic acid interactions. We have reported a novel method termed CRUIS (CRISPR-based RNA-United Interacting System), which captures RNA-protein interactions in living cells by combining the RNA-binding capacity of CRISPR/Cas13 and the proximity-tagging activity of PUP-IT. Enzymatically deactivated Cas13a (dCas13a) is fused to the proximity labeling enzyme PafA. In the presence of a guide RNA, dCas13a binds specific target RNA region, while the fused PafA mediates the labeling of biotin-tagged Pup on proximal proteins. The labeled proteins can be enriched by streptavidin pull-down and identified by mass spectrometry. Here we describe the ge... More

關鍵詞

CRISPR, CRUIS, PUP-IT, Proximity-based protein labeling, RNA-binding protein
              主站蜘蛛池模板: 沙田区| 蓬溪县| 樟树市| 南雄市| 灯塔市| 孟连| 耒阳市| 山丹县| 广州市| 扶绥县| 华池县| 大洼县| 文登市| 儋州市| 岢岚县| 天等县| 乌兰察布市| 吉水县| 许昌市| 闻喜县| 利辛县| 丰都县| 张掖市| 莆田市| 怀来县| 达拉特旗| 沾化县| 科技| 沈阳市| 梓潼县| 蛟河市| 南岸区| 炎陵县| 津南区| 水富县| 巫山县| 西安市| 固始县| 伊春市| 呼图壁县| 台北县|