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A novel and sensitive real-time PCR system for universal detection of poxviruses

Sci Rep. 2021-01;

Léa Luciani, Lucia Inchauste, Olivier Ferraris, Rémi Charrel, Antoine Nougairède, Géraldine Piorkowski, Christophe Peyrefitte, Stéphane Bertagnoli, Xavier de Lamballerie, Stéphane Priet

Products/Services Used	Details	Operation
Catalog Antibody	… Quantified synthetic plasmids (GenScript) containing the target sequence from the Monkeypox virus D6R orthologous genes were used as standards. Confirmed poxviral DNA samples were obtained from the National Reference Centre for Orthopoxvirus (France), the Animal …	Get A Quote

摘要

Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emerging poxviruses, requiring new improved diagnostic and epidemiological tools. We describe here a real-time PCR assay targeting a highly conserved region of the poxvirus genome, thus allowing a pan-Poxvirus detection (Chordopoxvirinae and Entomopoxvirinae). This system is specific (99.8% for vertebrate samples and 99.7% for arthropods samples), sensitive (100% for vertebrate samples and 86.3% for arthropods samples) and... More