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Screening of potent neutralizing antibodies against SARS-CoV-2 using convalescent patients-derived phage-display libraries

Cell Discov. 2021-07; 
Yongbing Pan, Jianhui Du, Jia Liu, Hai Wu, Fang Gui, Nan Zhang, Xiaojie Deng, Gang Song, Yufeng Li, Jia Lu, Xiaoli Wu, ShanShan Zhan, Zhaofei Jing, Jiong Wang, Yimin Yang, Jianbang Liu, Ying Chen, Qin Chen, Huanyu Zhang, Hengrui Hu, Kai Duan, Manli Wang, Qisheng Wang, Xiaoming Yang
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Catalog Antibody … Plates were then washed with PBST and incubated with a 1:3000 dilution of biotin-labeled anti-His antibody (Genscript, A00613) for 1 h at RT. After washing, 1:20,000 streptavidin–HRP (Invitrogen, SNN1004) was added to the plates and incubated for 1 h at RT. After washing … Get A Quote

摘要

As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to threaten public health worldwide, the development of effective interventions is urgently needed. Neutralizing antibodies (nAbs) have great potential for the prevention and treatment of SARS-CoV-2 infection. In this study, ten nAbs were isolated from two phage-display immune libraries constructed from the pooled PBMCs of eight COVID-19 convalescent patients. Eight of them, consisting of heavy chains encoded by the immunoglobulin heavy-chain gene-variable region (IGHV)3-66 or IGHV3-53 genes, recognized the same epitope on the receptor-binding domain (RBD), while the remaining two bound to different epitopes. Among the ten antibodies,... More

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