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3D culture system to study collective migration in mammary organoid epithelium

STAR Protoc. 2021-08; 
Yunzhe Lu, Ruolan Deng, Huanyang You, Pengfei Lu
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Recombinant Proteins We have compared FGF10 provided by different manufacturers and have found that rmFGF10 (GenScript, Cat#Z03155) worked well. Get A Quote

摘要

We recently established an culture system in which mammary gland organoid undergoes directional migration in response to an FGF10 concentration gradient. Here, we describe a step-by-step protocol for preparing organoids, the setup of the 3D culture system, and the image acquisition approach. The technical difficulties in conducting the 3D migration assay are choosing epithelial organoids of appropriate sizes and manually paring organoids and beads pre-soaked in FGF10 within a desirable distance (~100?μm). For complete details on the use and execution of this protocol, please refer to Lu et?al. (2020).

關(guān)鍵詞

Cell Biology, Cell culture, Developmental biology, Microscopy, Organoids
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