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Improved modeling of human AD with an automated culturing platform for iPSC neurons, astrocytes and microglia

Nat Commun. 2021-09; 
Reina Bassil, Kenneth Shields, Kevin Granger, Ivan Zein, Shirley Ng, Ben Chih
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Molecular Biology Reagents … The supernatant was removed and the remaining sarkosyl insoluble pellet was resuspended in sample buffer 4x LDS (Genscript M00676) with 5% beta-mercaptoethanol before electrophoresis. The soluble fraction was assayed by Micro BCA Protein Assay Kit (ThermoFisher #… Get A Quote

摘要

Advancement in human induced pluripotent stem cell (iPSC) neuron and microglial differentiation protocols allow for disease modeling using physiologically relevant cells. However, iPSC differentiation and culturing protocols have posed challenges to maintaining consistency. Here, we generated an automated, consistent, and long-term culturing platform of human iPSC neurons, astrocytes, and microglia. Using this platform we generated a iPSC AD model using human derived cells, which showed signs of Aβ plaques, dystrophic neurites around plaques, synapse loss, dendrite retraction, axon fragmentation, phospho-Tau induction, and neuronal cell death in one model. We showed that the human iPSC microglia internalized a... More

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