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Engineering Cupriavidus necator DSM 545 for the one-step conversion of starchy waste into polyhydroxyalkanoates

Bioresour Technol. 2021-11;

Silvia Brojanigo, Nicoletta Gronchi, Tiziano Cazzorla, Tuck Seng Wong, Marina Basaglia, Lorenzo Favaro, Sergio Casella

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Codon Optimization	… profunda SM-A87 (Qin et al., 2014) were codon-optimised and synthesised by GenScript (Piscataway, USA). The sequences were predicted to include a signal peptide for protein secretion. The gene encoding red fluorescent protein (RFP) was excised from P g25 [D1C2] -RFP …	Get A Quote

摘要

Starch-rich by-products could be efficiently exploited for polyhydroxyalkanoates (PHAs) production. Unfortunately, Cupriavidus necator DSM 545, one of the most efficient PHAs producers, is not able to grow on starch. In this study, a recombinant amylolytic strain of C. necator DSM 545 was developed for the one-step PHAs production from starchy residues, such as broken rice and purple sweet potato waste. The glucodextranase G1d from Arthrobacter globiformis I42 and the α-amylase amyZ from Zunongwangia profunda SM-A87 were co-expressed into C. necator DSM 545. The recombinant C. necator DSM 545 #11, selected for its promising hydrolytic activity, produced high biomass levels with noteworthy PHAs titers: 5.78 and... More