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Autophagy Activated by Peroxiredoxin of

Cells. 2020-11; 
Xia Li, Yuhan Zhang, Yanqing Zhao, Ke Qiao, Meng Feng, Hang Zhou, Hiroshi Tachibana, Xunjia Cheng
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Recombinant Antibody Expression … was used to purify the recombinant proteins, which were dialyzed against Tris-HCl (20 mM, pH 8.0), followed by filtration via a 0.22-μm membrane, and tested for endotoxin levels using the Toxin Sensor TM Gel Clot Endotoxin Assay Kit (Genscript, Nanjing, China, L00351)?… Get A Quote

摘要

Autophagy, an evolutionarily conserved mechanism to remove redundant or dangerous cellular components, plays an important role in innate immunity and defense against pathogens, which, in turn, can regulate autophagy to establish infection within a host. However, for , an intestinal protozoan parasite causing human amoebic colitis, the interaction with the host cell autophagy mechanism has not been investigated. In this study, we found that peroxiredoxin (Prx), an antioxidant enzyme critical for parasite survival during the invasion of host tissues, could activate autophagy in macrophages. The formation of autophagosomes in macrophages treated with recombinant Prx of for 24 h was revealed by immunofluorescence... More

關鍵詞

Entamoeba histolytica, autophagy, innate immunity, macrophage, peroxiredoxin, toll-like receptor 4
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