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A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions

G3 (Bethesda). 2020-08-01;

Dae-Kyum Kim, Jennifer J Knapp, Da Kuang, Aditya Chawla, Patricia Cassonnet, Hunsang Lee, Dayag Sheykhkarimli, Payman Samavarchi-Tehrani, Hala Abdouni, Ashyad Rayhan, Roujia Li, Oxana Pogoutse, étienne Coyaud, Sylvie van der Werf, Caroline Demeret, Anne-Claude Gingras, Mikko Taipale, Brian Raught, Yves Jacob, Frederick P Roth

Products/Services Used	Details	Operation
Codon Optimization	… MN975262; Chan et al. 2020) and Wuhan-Hu-1 (GenBank MN908947; Wu et al. 2020) isolates 82 of SARS-CoV-2, we requested the synthesis of viral coding sequences (GenScript and 83 Integrated DNA Technologies), including termination codons and attB recombination 84 …	Get A Quote

摘要

The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.