| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | BAP1 (C-4; sc-28383), FOXK1 (G-4; sc-373810), YY1 (sc-7341) and RAR-alpha (sc-551X) were purchased from Santa-Cruz; FLAG (M2; F1804) was purchased from Sigma; HCFC1 (A301–400A) and RNF2 (A302–869A) were purchased from Bethyl Laboratories; Lamin B1 (ab16048); H2AK119ub (D27C4; 8240S), H3K27me3 (C36B11, 9733S), H3K4me3 (C42D8, 9751) were purchased from Cell Signaling; H2B (5HH2–2A8; 61037); H2BK120Ub (C56; 39623), H3 (C-terminal; 39163), KDM1B (61457) were purchased from Active Motif; H3K4me2 (MCA-MAB10003–100-Ex) was purchased from Cosmo Bio; alpha-Tubulin (1F4E3; A01410) was purchased from Genscript. | Get A Quote |
In?Drosophila, a complex consisting of Calypso and ASX catalyzes H2A deubiquitination and has been reported to act as part of the Polycomb machinery in transcriptional silencing. The mammalian homologs of these proteins (BAP1 and ASXL1/2/3, respectively), are frequently mutated in various cancer types, yet their precise functions remain unclear. Using an integrative approach based on isogenic cell lines generated with CRISPR/Cas9, we uncover an unanticipated role for BAP1 in gene activation. This function requires the assembly of an enzymatically active BAPl-associated core complex (BAP1.com) containing one of the redundant ASXL proteins. We investigated the mechanism underlying BAP1.com-mediated transcription... More
