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Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms

Anti-diarrhoeal activity of polyherbal formulation. 2019; 
Sasikumar Murugan Divya Purusothaman Edwin Jothie Richard Nehru Sai Suresh Chalichem Bharathi Bethapudi Prasanna Raja Chandrasekaran Chandrasekaran Chinampudur Velusami Prashanth D’Souza Deepak Mundkinajeddu
Products/Services Used Details Operation
Recombinant Proteins HT-29 cells were procured from American Type Culture Collection (Manassas, USA) and maintained as cryopreserved stocks in liquid nitrogen containers (-196°C); ELISA kit (GenScript, NJ, USA); DMSO (Ranchem, India), MTT (Sigma, India), Forskolin (Sigma, India), McCoy’s 5A (Thermofisher scientific, US) were procured. Sodium dihydrogen phosphate anhydrase, calcium chloride, Sodium chloride, sodium bicarbonate, dextrose anhydrous, potassium chloride and magnesium chloride were procured from HiMedia Laboratories Pvt. Ltd., Mumbai, India. Automatic organ bath (PANLAB, Spain) and Chart 5 software (V5.5.6 AD instruments, New Zealand) were used for measuring ileal contractions. Get A Quote

摘要

Objective: The present study was aimed to study anti-diarrhoeal activity of a polyherbal formulation (PHF) in rats and elucidate its mechanism of action. Materials and Methods: Anti-diarrhoeal activity of PHF was investigated using castor oil-induced diarrhoea, small intestinal transit and enteropooling models in rats. PHF was tested at 75, 150 and 300 mg/kg rat body weight. Loperamide was used as a reference control for in vivo studies. Anti-secretory action was evaluated against heat labile enterotoxin (from Escherichia coli)induced secretion in rat ileal loop model. The effect of PHF (12.5-100 μg/ml) on cAMP-dependent secretory activity was investigated against forskolin-induced cAMP release in HT-29 cells.... More

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